Isabella LaFreniere was born in Lambertville, Michigan, and began her dance training at the age of seven at the Northeast Academy of Dance under Giuseppe Canale and Scott Heinrich. At 11, she began studying with Anna Reznik and Alexei Kremnev, first at the Southold Dance Theatre in South Bend, Indiana, and then at the Joffrey Academy of Dance in Chicago, Illinois.

She began studying at the School of American Ballet (SAB), the official school of New York City Ballet, during the 2008 summer course, and enrolled as a full-time student during the 2010 winter term. She received additional training at the Chautauqua Institution and Pacific Northwest Ballet summer programs.

Ms. LaFreniere became an apprentice with NYCB in August 2013 and joined the Company as a member of the corps de ballet in June 2014.

Ms. LaFreniere was promoted to the rank of soloist dancer in January 2022 and principal dancer in February 2023.